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Metabolic assays For real-time measurement of the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR), 2 10 5 in-vitro-polarized T H 1 cells were washed with Seahorse XF RPMI medium (pH 7.4) supplemented with 5 mM glucose (or without), 1 mM pyruvate, 2 mM glutamine, 100 g ml 1 linoleic or oleic acid and 5 mM NaCl or BHB, respectively

The starting dose is usually set at 0.25 mg once weekly for 4 weeks

Dec 2018;33(6):589-601

For a 100 mg target dose at 100 mg/mL: Volume to Draw = Desired DoseConcentration = 100 mg100 mg/mL = 1.0 mL Convert to syringe units