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Despite the thermodynamically highly favorable reverse reaction, PfGrx C88S (S 2 ) reacted very slowly with roGFP2 WT (SH) 2 most likely due to the instability and high energy of the Grx(SSR) intermediate
Once frozen, the peptide is ruined
The hepatic tissues were sectioned with a thickness of 5 m and deparaffinized using xylene
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