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Production of reactive oxygen species (ROS) was monitored following 5-(and-6)-chloromethyl-2,7-dichlorodihydrofluorescein diacetate acetyl ester and MitoSOX Redderived fluorescence

In addition to acidification, lactate is known to induce histone modification referred to as lactylation, which is associated with enhanced polarization of M2 cells, suppressed T cell effector functions and increased Treg differentiation, thereby supporting an anti-immune and pro-tumorigenic phenotype (153155)

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