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All optimized cut-off value were selected based on the Youden Index (YI) and were provided in Supplementary section

Luciferase reporter assays A bicistronic vector pcDNA3 RLuc POLIRES FLuc (empty reporter) (Addgene, 45642) and pcDNA3 RLuc POLIRES FLuc containing human PRKAA1 5UTR upstream of FLuc (5UTR reporter) were transfected into shCtrl and shGDH1 or shYBX1 Huh7 cells using Lipofectamine 2000 (Thermo Fisher)

Inhibition of D1 and D2 due to inflammation is the most common cause of low T3 but D3 is also a contributing factor to low T3 and increased rT3

We recommend N/A of bacteriostatic water, though anywhere between 1 mL and 3 mL is acceptable

It binds with high affinity to HGF inducing dimerization and Met phosphorylation in the presence of subthreshold levels of HGF [Figure 2] [74]