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After repeated washing the bound fraction was eluted with 250 mM imidazole (Fischer) in lysis buffer containing 10% glycerol (Acros)
The AUC ratio of glucuronide to acetaminophen increased with gestational age, that of sulfate decreased, and the ratio of cysteine and mercapturate remained unchanged
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The study used a single high dose and did not evaluate symptoms, disease outcomes or long-term use
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