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Ferroptotic stress promotes the accumulation of pro-inflammatory proximal tubular cells in maladaptive renal repair

Activation of nuclear factor-B (NF-B) induces transcription of target genes that primarily encode survival proteins (e.g., cellular FLICE-like inhibitory protein and inhibitor of apoptosis proteins) via formation of the complex I signalosome after binding to TNF receptor-1

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The membranes were blocked for 1 h in 5% skim milk and incubated overnight at 4 C with primary antibodies