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Cells were passaged at confluence with 0.05% trypsin and maintained in a 37 C incubator with humidified atmosphere of 5% CO 2 and 95% air
The preparation temperature must be lower than 40C
That role is real and well described in biochemistry
Study Significance of IGF-1 A deregulation or imbalance in the IGF system could have implications in a number of different disorders including perturbations with normal growth, cancer and diabetes
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