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THP-1, U2OS and Jurkat cells were stained with 1:100 FITC Annexin V (BioLegend, 640906) and 1:1,000 7-AAD (BioLegend, 420403)

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Nutrient Deficiencies (Building Blocks Missing) Glutathione synthesis requires specific nutrients: Cysteine : From dietary protein (whey, eggs, poultry)-rate-limiting Glycine : Often deficient in modern diets (need 10-15g/day, most get 3-5g) Selenium : Cofactor for glutathione peroxidase (GPx) , the enzyme that recycles GSSG back to GSH B vitamins (B6, B9, B12): Required for methylation and sulfur metabolism Vitamin C : Recycles oxidized glutathione Deficiency in any of these impairs glutathione production or recycling [5]

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Then the rats were administered with phenol red suspension