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The peptide was acquired as a 99% pure substance that was further dissolved in saline prior to the random assignment of male mice to an experimental treatment

However in the 3-D culture medium, adding IGF-1 can enhance the production of mature oocytes, and injection of IGF-1 before ovulation induction will increase the count of retrieved oocytes [17]

Lancet 363 , 21392141 (2004)

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