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88 Tumor-intrinsic PD-L1 amplifies JAK2/STAT3 signaling by sequestering PTP1B, a phosphatase that normally deactivates JAK2
Insulin/IGF1 receptor hybrid activation assay by immunoprecipitation and Western blot analysis was performed by addition of 30 l of Protein G-Agarose (Roche Diagnostic, Indianapolis, IN, USA) in 300 l buffer (100 mM Hepes, pH 7.8, 100 mM NaCl, 10 mM MgSO 4 , 0.02% Tween-20) and 1 g of the appropriate antibody for 3 hours at 4C followed by brief centrifugation
Activated MEK1/2 then activates ERK1/2 (MAPK)
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