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Although physicochemical characterization demonstrated appropriate vesicle size distribution, polydispersity index, zeta potential, and formulation stability, and the increase in vesicle size after RSV incorporation supported the association of RSV with the liposomal system, these parameters do not replace direct quantification of encapsulation efficiency

Lactated Ringers solution was provided intravenously at 10 mL kg per hour throughout anesthesia

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