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Following 24, 48, 72, or 96 h after seeding, the medium was supplemented with 10 L of CCK-8 reagent (Beyotime) and the cells were incubated at 37 C for 2 h

Statistical Analysis Statistical analysis was performed by parametric one-way analysis of variance (ANOVA), with the NewmanKeuls post hoc test or the non-parametric KruskalWallis test and subsequently the MannWhitney U test to compare groups

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