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Comprehensive genomic profiling using a hybrid capture-based DNA+RNA sequencing platform (Caris Life Sciences) revealed a PRKAR1A p.S321fs* pathogenic frameshift mutation (RefSeq transcript NM_001276289.1
The biomarkers of oxidative stress such as superoxide dismutase and malondialdehyde were significantly lessened in the test group
All restriction enzymes, Phusion DNA polymerase, and T4 DNA ligase were from New England Biolabs (NEB)
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In cases where multiple studies originated from the same cohort, the study with the larger sample size was selected
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