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Samples (30 g protein) were resolved by 420% SDS-PAGE (Biofuraw Precast Gel, Tanon Science, Shanghai, China), transferred to polyvinylidene difluoride membranes, and incubated with one of the following antibodies: LC3 (88588s, Cell Signaling Technology, 1:1000), GPX4 (ab125066, Abcam, 1:1000), p16 (1003-1-AP, Proteintech, 1:1000)

Glutathione injections contain L-Glutathione , a powerful antioxidant composed of amino acids

Eur J Biochem 267:19851994 Stephens FB, Constantin-Teodosiu D, Laithwaite D, Simpson EJ, Greenhaff PL (2006) An acute increase in skeletal muscle carnitine content alters fuel metabolism in resting human skeletal muscle

Acknowledgments This research was conducted with the support of the Office of Research at Shahid Sadoughi University of Medical Sciences