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After the reaction was complete, 48 kg of isopropyl acetate at room temperature was added to the reactor and the resulting mixture was maintained for 1 hour at 43-48 with moderate agitation, followed by filtration of the reaction mixture to remove the solids (Tyvek filter, 15.5" diameter, Kavon Filter Products, Wall, NJ, model No

The core principles are straightforward: lyophilized powder wants to be cold, dry, dark, and sealed

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Hydration matters before and after the session

Slides were counterstained with DAPI and coverslipped with Prolong TM Gold Antifade mountant (Thermo Fisher Scientific)