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Peptide purity is verified through high-performance liquid chromatography (HPLC), confirming compound concentration and the absence of significant impurities

Several groups face higher risk, including: Older adults (due to reduced stomach acid production) Vegans and vegetarians (limited dietary sources) Individuals with pernicious anaemia (an autoimmune condition affecting B12 absorption) People with gastrointestinal disorders such as Crohn's disease or coeliac disease Those who have undergone gastric surgery People taking certain medications long-term, particularly metformin and proton pump inhibitors Individuals with recreational or medical nitrous oxide exposure (which can inactivate B12 and cause acute neurological symptoms) Vegans and vegetarians should ensure adequate B12 intake through fortified foods (such as some plant milks, breakfast cereals, and yeast extracts) or supplements