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To prepare a 20-l solubilized mitochondrial sample, 20 g of mitochondrial protein (adjusted to 13 l in HB) was mixed with 5 l of 4 sampling buffer (200 mM Bis-Tris, 68 mM HCl, 200 mM NaCl) and 2 l of 10% digitonin buffer (0.2 mg digitonin in 2 l of water)
The point is, we just dont know, and there's so much more research that needs to be done
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(2024) Evaluating the impact of testosterone replacement therapy on carotid atherosclerosis: a systematic review and meta-analysis Haider SH, Irfan A, Sheikh SM, et al
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