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Candidates for research or therapeutic application include those with primary or secondary insomnia, difficulty initiating or maintaining sleep, or disrupted circadian rhythms

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Standard research protocols involve nasal spray administration , which provides direct CNS access

Cells treated with test compounds were washed with PBS and incubated with 10 M 2,7-dichlorofluorescein diacetate (DCFH-DA) at 37 C for 30 min, followed by washing with PBS, digestion with trypsin, and collection

Unlike IV infusion, subcutaneous administration eliminates the flushing, nausea, and chest tightness that IV protocols can produce when infused too rapidly