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Preclinical results do not automatically translate to human safety
For gut patients, the timeline is even faster
BPC-157 is an experimental peptide, and it has not been approved by the FDA to diagnose, treat, cure, or prevent any disease
For IGF-1R/InsR HybridR activation, IGF-1R immunoprecipitates were probed with an antibody against the InsR specific pTyr 1334 residue (#44809G, Invitrogen/ThermoFisher) 52 , and total HybridR determined by immunoblotting with an Anti-InsR antibody (#3025)
Rat beta-NGF DuoSet ELISA, R&D Systems, Cat# AF-556-NA) were analysed in the posterior cortex by enzyme-bound immunosorbent assay (ELISA) in a microplate photometer using commercial kits (Peprotech, So Paulo, Brazil)
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