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Cycle threshold (Ct) values of target genes were calibrated to the Ct for the corresponding genes obtained for samples from the control pCYC1-GAP1 strain and normalised to the geometric mean of the reference genes for relative quantification using the comparative Ct method [36]

(4) This interaction may be crucial as FOXO4, when bound to p53, may inhibit some of p53's key functions involved in cell cycle regulation and apoptosis