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Forty to 48 h after transfection, supernatant was collected into 50-ml Falcon tubes and centrifuged at 500 g for 5 min to pellet cell debris
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In addition, they found that both the transcript and the protein levels of Nrf2 and the detoxifying/antioxidant enzymes HO1 and NQO1 were significantly decreased [252]
Favorable results after conservative management of 316 valproate intoxicated patients
