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Prolonged immobilization leads to scar tissue formation that limits the range of motion and increases re-injury risk
This allows multiple withdrawals across weeks of treatment
The very low rate constants for direct, glutathione-independent dithiol-disulfide exchange reactions between glutaredoxins and roGFP2 or other protein(S 2 )/protein(SH) 2 couples (despite favorable thermodynamics) are a prerequisite and great advantage for reliable redox measurements in vivo
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