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Cells were spun at 4C for 20 min to pellet, and resuspended in lysis buffer (50 mM NaH2PO4, 300 mM NaCl, 10 mM imidazole, pH = 8)

10.1002/chem.201406236 64 SinningA.HbnerC

NACC, whose mechanism of action remains unclear, demonstrated selective anticancer activity, and low toxicity, which make NACC and its analogs promising anticancer agents ( Another approach was to use the sulphonamidase activity of GST

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