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For experiments involving caspase-3/7 staining, CellEvent Caspase-3/7 Green Detection Reagent (Thermo Scientific C10723) and CPP-CAND were added to live cells 24 h before imaging using an Eclipse Ti-U inverted microscope (NIKON)

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There is slower migration of fibroblasts and wound-healing cells to these sites of injury owing to the poor blood supply, and therefore the repair process is obstructed