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GLP-1R-SmBiT V229A was generated from the WT template by site-directed mutagenesis using the following primers: Forward (5 to 3): TCTTTCCTGTCGACTCGCGTTTCTGCTTATGCAGT, Reverse (5 to 3): ACTGCATAAGCAGAAACGCGAGTCGACAGGAAAG
Effects of losartan on renal and cardiovascular outcomes in patients with type 2 diabetes and nephropathy
It is naturally present in human blood, saliva, urine, and its plasma concentration declines with age from approximately 200 ng/mL at age 20 to under 80 ng/mL after age 60
It appears that some small peptides (chains of 2 or 3 amino acids) can be absorbed without further reduction
The human monocytic leukemia cell line THP-1 (Bio-Channel, Nanjing, China) was cultured in RPMI-1640 medium supplemented with 10% heat-inactivated FBS, 1% penicillin-streptomycin (Bio-Channel, Nanjing, China), and 0.05 mM -mercaptoethanol (Merck, Buchs, Germany)
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