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More serious B12 deficiencies have the potential to impair heart and neurological function, leading to a wide range of serious symptoms, including but not limited to: tinnitus, severe joint pain, memory problems, depression, anxiety, poor muscle function, ataxia and changes in reflexes

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One g of RNA was reverse transcribed into cDNA using the Transcriptor First Strand cDNA synthesis kit (Roche, Indianapolis, IN, USA) and subjected to quantitative PCR using the SYBR-Green Supermix (Roche, Indianapolis, IN, USA) with the ABI Stepone Plus real-time PCR detection system (Applied Biosystems, Foster City, CA, USA)

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Here are a few potential drawbacks to consider: Possible Adverse Reactions Some individuals might experience mild side effects at the injection site, such as redness, swelling, or a sense of discomfort