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BDNF knockdown experiments further cement its role: mice with conditional BDNF deletion in the ventral tegmental area (VTA) or hippocampus develop spontaneous depressive phenotypes, even in the absence of ethanol, while BDNF infusion into these regions rescues ethanol-induced anhedonia (Zhong et al

[80] [81] [82] Other trials have confirmed reductions in symptoms, within 6 months in one trial, an effect that was maintained after withdrawal of the alpha blocker

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Hitting exactly 16.7 units on a syringe is not realistic, so you round to the nearest whole unit and accept a small margin of variation