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Analysis of reactive oxygen species (ROS) Intracellular ROS levels were measured using the oxidation-sensitive fluorescence probe DCFH-DA, following the manufacturers instructions (Beyotime, #S0033S, China)

Whilst we found that melatonin did not affect the concentration of G6P ( Figure 5H ), it did seem to greatly reduce the amount of F6P present in our cell samples, indicating potentially that the key disruption to this pathway occurred at the step where G6P is converted to F6P ( Figure 5E )

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Oocytes were mounted on a glass slide in a 1 L drop of SlowFade with 5 g/mL Hoechst 33,342 under a coverslip to visualize chromosomes