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Anyone with a history of cancer should regard pro-angiogenic peptides with particular caution

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For Zebrafish larvae, a 5 M DCFDA solution was used for staining

However, reports suggest that ROS can cause cytoskeletal remodeling in endothelial cells, leading to increased endothelial permeability due to altered calcium and cyclic adenosine monophosphate (cAMP) levels 27