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In the SKOV-3 cell line extending the BSO pretreatment period to 48 h from 24 h marginally reduced the cytotoxicity of cisplatin, whereas the cytotoxicity of the other three drugs remained similar to that observed after 24 h BSO pretreatment
The pellet was resuspended in 200 l of 8 M urea with 10 mM Tris (pH 8), incubated at 60 C for 30 min with shaking and centrifuged for 15 min at 18,000 g for protein quantification of the supernatant with the DC Protein Assay (Bio-Rad Laboratories)
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Selenium supplementation for patients with Graves hyperthyroidism (the GRASS trial): study protocol for a randomized controlled trial
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