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What the research does not show is a strong dose-response curve beyond 10 mg
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The cells were harvested by centrifugation (1000 x g , 2 min) and resuspended in lysis buffer (5% w/v 5-sulfosalicylic acid in 0.1 M potassium phosphate buffer with 5 mM EDTA (KPE buffer [35])) together with 425600 m-diameter acid-washed glass beads (Sigma) at a buffer/bead ratio of 1:2 by volume
Interleukin-1 antagonism in type 1 diabetes of recent onset: two multicentre, randomised, double-blind, placebo-controlled trials
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