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For GST pulldowns, researchers usually first purify the GST-fusion version of their protein of interest
In these cases, chromogenic anti-factor Xa assays can be used to measure the anticoagulant effect of factor Xa inhibitors (e.g., apixaban, rivaroxaban, edoxaban)
doi: 10.1101/gad.436307 89 GaoDNolanDJMellickASBambinoKMcDonnellKMittalVet al
RNA isolation and qRT-PCR Total RNA was extracted from primary astrocytes and striatum tissue using TRIzol reagent (Invitrogen, 15596026) and reversed transcribed into cDNA using PrimeScript RT Master Mix (Takara, RR036A)
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