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Cell pellets were then lysed in 50 l of 5% SDS, 100 mM TEAB and sonicated (QSonica) for 5 cycles, with a 1 min resting period on ice after each cycle
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This functional disorder not only disrupts the normal regulatory mechanisms of blood vessels but also leads to increased blood pressure, arterial stiffness, and remodeling of small arteries, ultimately resulting in multi-organ damage (Boutouyrie et al., 2021
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