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2024;154(1):e2023064167
Liver Digest Medium: HBSS, calcium, magnesium, no phenol red, 25 mM HEPES, penicillin-streptomycin (10,000 U/mL), 3.6 mg Trypsin Inhibitor (Sigma, St
Serum limitation-induced senescent fibroblasts model and effect of FOXO4-DRI Passage 3 fibroblasts derived from keloids and normal skins were cultured in a Dulbeccos Modified Eagles medium (DMEM, Hyclone, Logan, Utah, USA) containing 10% fetal bovine serum (FBS, Gibco, Grand Island, NY, USA) to 90% confluence, and then cultured in a DMEM containing 2% FBS for 3 days to construct a serum limitation-induced senescent fibroblast model