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Quantitative reverse transcription PCR (RT-qPCR) Total RNA was isolated from cells or tumor tissues using an RNA-easy Isolation Reagent (R701-01, Vazyme, China)

[28] [29] Testosterone promotes prostate cell proliferation, [30] but relatively low levels of serum testosterone are found in patients with BPH

These reactions are often temporary, but patients should continue monitoring the area and contact their healthcare provider when symptoms are severe, worsening, or do not improve

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