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For the measurement of hypotaurine, taurine, and cystathionine, the prepared homogenate or medium was mixed with one volume of 5 % (wt/vol) sulfosalicylic acid, and the mixture was centrifuged at 15,000 g for 15 min at 4 C to obtain the acid supernatant
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Then do the same for the bacteriostatic water vial
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