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For a worked walkthrough, see our sample COA documentation

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For nuclear, membrane, chromatin-associated, or low-copy targets, enrich the appropriate fraction first

IFIH1 encodes melanoma differentiation-associated protein 5 (MDA5), a ubiquitously expressed cytoplasmic RNA helicase and sensor that is crucial to survival after systemic viral infection by inducing an MAVS-mediated type I IFN immune response (98)