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Frozen cortical samples (the weights of which were determined beforehand) were homogenized in 5% 5-sulfosalicylic acid (SSA), and the mixtures were centrifuged at 8000 g for 10 min at 4 C

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The ideal temperature for storage is 25 degrees centigrade

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Although the acute intravenous administration of amlodipine decreases arterial blood pressure and increases heart rate in hemodynamic studies of patients with chronic stable angina, chronic oral administration of amlodipine in clinical trials did not lead to clinically significant changes in heart rate or blood pressures in normotensive patients with angina