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For immunofluorescence, sections were washed with PBS at room temperature twice for 10 min, followed by incubation in blocking buffer (5% fish gelatine, 0.5% Triton in PBS) at room temperature for 1 h before incubation with the primary antibodies (see Supplementary Table 4 for primary antibodies used in this study) diluted in the blocking buffer at 4 C overnight

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This natural origin is part of why GHK-Cu is a recurring subject in research on tissue remodeling and cellular aging, distinguishing it from fully synthetic peptides that lack a native counterpart in the body

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