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Leaner individuals may need a 1/2-inch needle to avoid hitting muscle
The blots were probed with the primary antibodies specific for phosphorylated PERK and CHOP (Thermo Fischer Scientific Inc.) at 4C overnight, then the blots were rinsed 35 times for 5 min with TBST and incubated with the horseradish peroxidase (HRP)-conjugated anti-rabbit IgG Secondary Antibody (Thermo Fischer Scientific Inc.) against the blotted target protein for 1 h at room temperature
For more details, please refer to the manual: Handling and Storage of Synthetic Peptides References Khavinson VKh, Bondarev IE, Butyugov AA
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