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MIN-6 cells were transfected with constructs encoding the human FLAG-tagged GLP-1R in the hygromycin-resistant vector pcDNA3.1 with Lipofectamine 2000 (Thermo Fisher Scientific, Massachusetts, USA) 3,8,43
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On average, surgical patients lost 58 pounds (around 24% of their total weight) by the end of year two, whereas GLP-1 patients on average lost 12 pounds (around 4.7% of their total weight)a fivefold difference
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