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Low-frequency resistance mutations are difficult to detect accurately, while background interference, such as clonal hematopoiesis, can readily lead to false-positive results

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The peptide is also known as [Nle4, D-Phe7]--MSH due to its specific modifications, which include the replacement of methionine with norleucine at the fourth position and the substitution of L-phenylalanine with D-phenylalanine at the seventh position [3]

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