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Ascorbate, dithiothreitol and TCEP reduce copper(II) to copper(I), and the donor set that stabilizes the divalent ion does not stabilize the monovalent one, so the complex is destroyed and the released copper becomes redox-active
10.3389/fnut.2020.00164 6
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The ratio of reduced glutathione to oxidized glutathione (the GSH:GSSG ratio) is one of the most critical clinical biomarkers for measuring cellular health and oxidative stress
