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For the surgical procedure, rats were anesthetized using ketamine (70 mg/kg, intramuscular (I.M.)) and xylazine (8 mg/kg, I.M.) (Salazar et al
After loading the crude solution that contained the target protein, some of the proteins are bounded
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To assess the potential impact of genome editing-mediated Exe4 secretion on endogenous hormone regulation, plasma GLP-1 levels were measured following an oral glucose challenge in saline- and Donor+Cas9-treated mice
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