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LC n3-PUFA showed a reduction without reaching the target (0.33, 6,000 mg/day), while vitamin B showed an opposite effect (+1.00 at 14 mg/day)

Cell pellets were resuspended in 0.3 ml small-scale lysis buffer (20 mM potassium phosphate, 200 mM NaCl, pH 7.4, 1 mg ml 1 lysozyme, 0.75 mg ml 1 polymyxin B, 0.01 mg ml 1 DNase I) per well and incubated at 30 C, 300 rpm, 5 cm shaking diameter for 30 min

Use an insulin syringe with a short needle (2831 gauge, 0.5 inch), pinch the skin, and insert at a 45-degree angle

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