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Serum-free or low-serum conditions are essential because serum IGFBPs in the media would partially sequester even LR3 at the highest serum concentrations

Consistent with prior reports that TRPA1 channels are expressed in STC-1 cells 8,9 , and that TRPA1 channel activation leads to GLP-1 secretion from this cell line 9 , the established TRPA1 channel activator allyl isothiocyanate (AITC) raised the [Ca 2+ ] i , and this effect was abrogated by A967079 (Fig