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The PCR products were gel-extracted, purified and cleaned (Macherey-Nagel, 740609) and 200 ng of each laccase mutant gene was transformed along with 100 ng of linearized vector into S
A 1:5 volume of chloroform was added, and the tube was vortexed and subjected to centrifugation at 12,000 g for 15 min
GSH also acts as the primary intracellular antioxidant, directly neutralizing reactive oxygen species (ROS) and protecting against lipid peroxidation, while serving as a cofactor for enzymes such as glutathione peroxidase (GPx) and glutathione reductase (GR)
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