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PMID: 29535770

The second is that a large share of the GHK corpus, including several of the most-cited review articles, traces back to a small number of investigators with continuous involvement in the field since the original isolation

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The positive transformants were selected on YPD plates with addition of antibiotics geneticin (G418, 4 mg/mL) or hygromycin B (HygB, 1 mg/mL) and verified by DNA sequencing after the integrated gene fragments were amplified by PCR using the corresponding primers and the genomic DNA templates