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The complex sample was concentrated and loaded onto SEC (SRT SEC-500 Sepax) in the buffer containing 20 mM Hepes pH 7.5 and 100 mM NaCl
However, its more commonly sold as an intramuscular injection
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Finally, we turned to targeting Tsa1, human PRDX3, and human PRDX5 (which are the two 2Cys peroxiredoxins residing in the matrix of human mitochondria) as well as human PRDX6 (a 1Cys peroxiredoxin found in the cytosol of human cells) to the mitochondrial matrix of prx1 cells (Appendix Fig S6A)
The 12:6 split reflects the typical relative quantities used when the two classes are studied together, with the GHRH-class compound as the larger fraction
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