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Mass spectrometry analysis of isotope labelled ([3- 13 C]Cholesterol, left) and unlabelled (right) cholesterol in A-498 UGDH _KO cells expressing a sgRNA resistant UGDH cDNA or an empty vector cultured in the presence of native LDL (grey) or 13 C-Cholesterol-labelled LDL for 4 h (dark blue) and 8 h (light blue)

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The coupling of an acetyl group to a glutathione molecule favors efficient uptake into cells